Background: The role and effects of people’s spiritual well-being have received more attention in recent years. Knowing the factors related to spiritual well-being, especially in students as the educated class and future builders of society, is too important. The aim of this study was to investigate the effectiveness of education on spirituality through social media in the spirituality well-being of public health students of Isfahan University of medical science.Methods: A semi-experimental, pre-test, post-test study was conducted on 50 under- graduate public health students (3 men, 47 women; age range 18-30 years) of Isfahan University of Medical Sciences through convenience and purposive sampling. The educational content of spirituality education was used to promote and improve spiritual well-being, being sent by using one of the mobile phone applications. Using spiritual well-being questionnaire, the level of the individuals’ spiritual well-being before and after the educational was evaluated. To analyze the data in this study, descriptive statistics and t-test were use SPSS software was used to analyze the data and the significance level was considered lower than 0.05%.Result: In total, 50 students including 3 men and 47women participated in the study. The mean age of the participants was 22.02±3.78. About 68% of the students were single and the remaining were married. The score of the participants’ spiritual well-being was 96.5 before the intervention and it promoted to 103.3 after the intervention. The result of the analysis by t-test on the two groups showed that spirituality education can cause a significant increase in peoples’ spiritual well-being (P<0.001). Conclusion: After the educational intervention, the level of people’s spiritual well-being increased significantly. As a result, spirituality education causes conditions to improve the peoples’ spiritual well-being.